Daily Archives: October 10, 2020

DNA Transfection and Luciferase Assay

DNA Transfection and Luciferase Assay The plasmid NF-kB containing the luciferase coding sequence under the control of NF-kB was kindly supplied by Dr. S. Roman. Cells were seeded at a cell density of 104 cells per well in 96-well plates and were transfected on the following day with plasmid NF-kB (Lipofectamine 2000; Invitrogen SA; Izasa Barcelona, Spain). To […]

In Vitro Model of LPS-induced Inflammation

We hypothesized that the antiinflammatory activity would be an important component of the therapeutic effect of TEL on respiratory infections. Thus, we designed the present study to test the antiinflammatory activity of TEL in an in vitro model of LPS-induced airway inflammation, and to determine whether this antiinflammatory activity occurs in vivo, in a mouse model of acute, LPS-induced […]

Airway inflammation is a common feature of infectious pulmonary diseases

Conclusions: We have provided evidence that TEL exerts potent antiinflammatory effects in LPS-induced airways injury. We propose that TEL acts in the early phase of inflammation by reducing the release of inflammatory mediators through NF-kB inhibition, and in the later phase through enhancement of inflammatory cell apoptosis. Key words: airway inflammation; antiinflammatory activity; apoptosis; macrophage inflammatory protein-2; […]